Activity Assay:Article Title: Characterizing the reproducibility in using a liver microphysiological system for assaying drug toxicity, metabolism, and accumulation
Article Snippet: .. Upon assaying effects of varying concentrations of the compounds troglitazone, tamoxifen and digoxin on LDH secretion, CYP3A4 activity and albumin production, the resulting data was submitted to a sigmoid curve (QuestGraphTM IC50 Calculator, AAT Bioquest, Inc., Sunnyvale, CA; URL: https://www.aatbio.com/tools/ec50-calculator) to estimate the concentration of each compound that induced half-maximal response (EC50) for the different assays.29 Labeling and imaging scaffolds with cells Once removed from the liver MPS, scaffolds with cells cultured in 3D were individually transferred to wells of a 24 well and washed once in PBS before being fixed in a solution of 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 minutes and washed three times in PBS. .. Then samples were permeabilized with a solution of 0.1% Triton X-100 (Sigma-Aldrich) in PBS, washed three times in PBS and incubated for 30 minutes in PBS containing Oregon Green 488 Phalloidin (Thermo Fisher Scientific) diluted 1:200 to label actin.
Concentration Assay:Article Title: Characterizing the reproducibility in using a liver microphysiological system for assaying drug toxicity, metabolism, and accumulation
Article Snippet: .. Upon assaying effects of varying concentrations of the compounds troglitazone, tamoxifen and digoxin on LDH secretion, CYP3A4 activity and albumin production, the resulting data was submitted to a sigmoid curve (QuestGraphTM IC50 Calculator, AAT Bioquest, Inc., Sunnyvale, CA; URL: https://www.aatbio.com/tools/ec50-calculator) to estimate the concentration of each compound that induced half-maximal response (EC50) for the different assays.29 Labeling and imaging scaffolds with cells Once removed from the liver MPS, scaffolds with cells cultured in 3D were individually transferred to wells of a 24 well and washed once in PBS before being fixed in a solution of 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 minutes and washed three times in PBS. .. Then samples were permeabilized with a solution of 0.1% Triton X-100 (Sigma-Aldrich) in PBS, washed three times in PBS and incubated for 30 minutes in PBS containing Oregon Green 488 Phalloidin (Thermo Fisher Scientific) diluted 1:200 to label actin.
Labeling:Article Title: Characterizing the reproducibility in using a liver microphysiological system for assaying drug toxicity, metabolism, and accumulation
Article Snippet: .. Upon assaying effects of varying concentrations of the compounds troglitazone, tamoxifen and digoxin on LDH secretion, CYP3A4 activity and albumin production, the resulting data was submitted to a sigmoid curve (QuestGraphTM IC50 Calculator, AAT Bioquest, Inc., Sunnyvale, CA; URL: https://www.aatbio.com/tools/ec50-calculator) to estimate the concentration of each compound that induced half-maximal response (EC50) for the different assays.29 Labeling and imaging scaffolds with cells Once removed from the liver MPS, scaffolds with cells cultured in 3D were individually transferred to wells of a 24 well and washed once in PBS before being fixed in a solution of 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 minutes and washed three times in PBS. .. Then samples were permeabilized with a solution of 0.1% Triton X-100 (Sigma-Aldrich) in PBS, washed three times in PBS and incubated for 30 minutes in PBS containing Oregon Green 488 Phalloidin (Thermo Fisher Scientific) diluted 1:200 to label actin.
Imaging:Article Title: Characterizing the reproducibility in using a liver microphysiological system for assaying drug toxicity, metabolism, and accumulation
Article Snippet: .. Upon assaying effects of varying concentrations of the compounds troglitazone, tamoxifen and digoxin on LDH secretion, CYP3A4 activity and albumin production, the resulting data was submitted to a sigmoid curve (QuestGraphTM IC50 Calculator, AAT Bioquest, Inc., Sunnyvale, CA; URL: https://www.aatbio.com/tools/ec50-calculator) to estimate the concentration of each compound that induced half-maximal response (EC50) for the different assays.29 Labeling and imaging scaffolds with cells Once removed from the liver MPS, scaffolds with cells cultured in 3D were individually transferred to wells of a 24 well and washed once in PBS before being fixed in a solution of 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 minutes and washed three times in PBS. .. Then samples were permeabilized with a solution of 0.1% Triton X-100 (Sigma-Aldrich) in PBS, washed three times in PBS and incubated for 30 minutes in PBS containing Oregon Green 488 Phalloidin (Thermo Fisher Scientific) diluted 1:200 to label actin.
Cell Culture:Article Title: Characterizing the reproducibility in using a liver microphysiological system for assaying drug toxicity, metabolism, and accumulation
Article Snippet: .. Upon assaying effects of varying concentrations of the compounds troglitazone, tamoxifen and digoxin on LDH secretion, CYP3A4 activity and albumin production, the resulting data was submitted to a sigmoid curve (QuestGraphTM IC50 Calculator, AAT Bioquest, Inc., Sunnyvale, CA; URL: https://www.aatbio.com/tools/ec50-calculator) to estimate the concentration of each compound that induced half-maximal response (EC50) for the different assays.29 Labeling and imaging scaffolds with cells Once removed from the liver MPS, scaffolds with cells cultured in 3D were individually transferred to wells of a 24 well and washed once in PBS before being fixed in a solution of 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 minutes and washed three times in PBS. .. Then samples were permeabilized with a solution of 0.1% Triton X-100 (Sigma-Aldrich) in PBS, washed three times in PBS and incubated for 30 minutes in PBS containing Oregon Green 488 Phalloidin (Thermo Fisher Scientific) diluted 1:200 to label actin.
Electron Microscopy:Article Title: Characterizing the reproducibility in using a liver microphysiological system for assaying drug toxicity, metabolism, and accumulation
Article Snippet: .. Upon assaying effects of varying concentrations of the compounds troglitazone, tamoxifen and digoxin on LDH secretion, CYP3A4 activity and albumin production, the resulting data was submitted to a sigmoid curve (QuestGraphTM IC50 Calculator, AAT Bioquest, Inc., Sunnyvale, CA; URL: https://www.aatbio.com/tools/ec50-calculator) to estimate the concentration of each compound that induced half-maximal response (EC50) for the different assays.29 Labeling and imaging scaffolds with cells Once removed from the liver MPS, scaffolds with cells cultured in 3D were individually transferred to wells of a 24 well and washed once in PBS before being fixed in a solution of 4% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 minutes and washed three times in PBS. .. Then samples were permeabilized with a solution of 0.1% Triton X-100 (Sigma-Aldrich) in PBS, washed three times in PBS and incubated for 30 minutes in PBS containing Oregon Green 488 Phalloidin (Thermo Fisher Scientific) diluted 1:200 to label actin.
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